Actinomadura madurae is an aerobic actinomycete which is best known worldwide as the cause of actinomycotic mycetomas. It has not previously been reported. Here, we utilized deep sequencing of 16S rRNA to implicate Actinomadura madurae as the cause of mycetoma in a diabetic patient when culture and. Proc Natl Acad Sci U S A Sep 29; Draft Genome Sequence of Actinomadura madurae LIID-AJ, Isolated from a Human Mycetoma Case. Vera-Cabrera L.

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Molecular Diagnosis of Actinomadura madurae Infection by 16S rRNA Deep Sequencing

Deep sequencing has previously been used to characterize cultured bacterial isolates 27 and to explore the composition of microbial populations in metagenomic research 28 A second biopsy specimen was submitted for culture and was again positive for S.

A number of other organisms were also detected, reflecting the polymicrobial nature of such specimens 5. The genus Actinomadura is one of four genera of actinobacteria that belong to the family Thermomonosporaceae. Despite treatment, the lesions continued to steadily grow, and 2 years prior to presentation she received a 6-week course of intravenous penicillin therapy with continuous daily infusions 20 million units intravenously [i.

Articles using diversity taxobox Articles with ‘species’ microformats All stub articles. Address correspondence to Stephen J. A portion of the specimen was concurrently submitted for culture in order to establish the identity of the aerobic actinomycetes-like organism and to evaluate its antibiotic sensitivities; however, the culture quickly became overgrown by Staphylococcus aureuspreventing culture for slower-growing aerobic actinomycetes species. This page was last edited on 25 Marchat Here, we have extended the application of next-generation DNA sequencing technologies to perform molecular diagnosis in a clinical context.


Comparison of species distribution and antimicrobial susceptibility of aerobic actinomycetes from clinical specimens.

DNA was amplified from the specimen in two separate reactions, in which sequencing adapters were incorporated on opposite ends of the amplicon, enabling bidirectional sequencing across the full length of 16S rRNA variable regions 1 and 2 3.

Mycetomap 80— In Rippon J, editor. The portion of the biopsy material sequenced may have contained little of the aerobic actinomycetes inclusion, which represented a focal area within the larger sample Fig.

Abstract Next-generation DNA sequencing can be used to catalog individual organisms within complex, polymicrobial specimens. This finding is consistent with the results of both culture and Sanger madurxe for the specimen, actinomaxura were dominated by S. Consistent with culture results, reads classified as S. Rapid 16S rRNA next-generation sequencing of polymicrobial clinical samples for diagnosis of complex bacterial infections. Based on this information, a course of sulfamethoxazole-trimethoprim therapy was considered.

Based on these findings, the diagnosis of actinomycotic mycetoma was suggested. Departments of Laboratory Medicine a.

Actinomadura madurae – Global Catalogue of Microorganisms

Base calling was performed using TorrentServer software, version actinommadura. Examination with next-generation sequencing technology of the bacterial microbiota in bronchoalveolar lavage samples after traumatic injury. Comparison of two next-generation sequencing technologies for resolving highly complex microbiota composition using tandem variable 16S rRNA gene regions.


Monsaasa Mimosa Chaua Lindley A. Here, we utilized deep sequencing of 16S rRNA to implicate Actinomadura madurae as the cause of mycetoma in a diabetic patient when culture and conventional molecular methods were overwhelmed by overgrowth of other organisms.

Actinomycetoma in the United States. Culture of discharged material reportedly returned with overgrowth of skin flora and provided no evidence of infection with Actinomyces species. Treatment of actinomycetoma foot—our experience with ten patients.

Actinomycetoma in the United States. Although the patient remained ambulatory throughout the course of her disease, she gradually had to modify her footwear to actinmoadura the increasing size of her foot, which at the time of presentation was causing her great pain.

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In this case report, we have utilized the approach to investigate the causative agent of biopsy-proven actinomycotic mycetoma. Next-generation sequencing in clinical microbiology.

J Lab Clin Med. Abstract Actinomadura madurae is an aerobic actinomycete which is best known worldwide as the cause of actinomycotic mycetomas. Actinomadura Lechevalier and Lechevalier Many sites of the human body are colonized by complex communities of microbes in both health and various disease states 6.

A Punch biopsy material stained using hematoxylin and eosin.